Experimental Studies on the Effects of Peristalsis upon the Movement of Orally taken Organisms in Dogs' Intestine

Abstract

Multiplication of orally taken organisms in the intestines has been widely discusseed in order to study the onset of intestinal infectious diseases.
As to the most fundamental aspects of this problem the following experiments were carried out.
(1) Separate cultures of contents of the stomach, the jejunum, the ileum and the transverse colon acquired by laparotomy at one and a half hours and 4 hours after oral administration of Escherichia freundii, Ballerup strain, resistent to 2500 r/ml of streptomycin, to different dogs revealed the following results. With no exceptions, in three cases of the one-and-a-half-hour group, the given organisms were all proved to remain in the stomach and the jejunum, while in the 4-hour group they were discovered to move down to the ileum and the large intestines and none were found in the upper tracts.
(2) Cultures done specimens taken at frequent intervals for 24 hours after direct injection of the same strain to the stomach by laparotomy under intravenous anesthesia with Ravonal revealed that the given organisms remained in the stomach and the jejunum all through 24 hours.
(3) When the organisms were injected to the ileum in the experiments similar to Part 2, they were proved to stay there at least for 6 hours.
(4) In several cases of experiments (1) and (2), organisms labelled with Isotop P₃₂ were administered, confirming the parallel relationship between the number of the living organisms and the degree of the radioactivity in the acquired specimens. However, since the number handled here was limitted, further investigations will be necessary to reach a definite conclusion in this matter.
In conclusion through these experiments it was felt that when normal peristalsis was maintained, orally taken organisms were transfered at considerable speed and that if peristalsis was diminished they either remained in the same place for a long period or was transferned extremely slowly.
Therefore, paralizing intestines is one of the useful means of transplantation of certain organisms in a proposed portion of the intestinal tracts.