1961 Volume 34 Issue 12 Pages 1211-1217
It was reported in the previous paper, that the rabbits immunized with Sh. dysenteriae 1 excreted in their stools a special active substance.
This substance tentatively named a special biologically active substance (SBAS) was demonstrated in stools not only of experimental animals but also of dysentery patients.
A method for demonstration of this substance in stools of Sh. flexneri 2a patients and a determination technic based on the variation of turbidity of the Sh. flex. 2a suspension were reported in this paper.
1) The suspension of Sh. flex. 2a treated with alcohol changed it's turbidity, when added with a minimum amount of the stool of Sh. flex. 2a patient.
2) The change of the turbidity most remarkably occurred at 43°.
3) At 43° the turbidity of the suspension continued to decrease for 15 hours, it's degree corresponding to the concentration of the stool.
4) The determination method of minimum amounts was devised from these properties of the substance mentioned above.