Studies on the Salmonellosis of Fowls with Special Reference to Diagnosis of S. Pullorum Carrier

II. Studies on the “intermittent type” of the pullorum disease in artificially infected fowls

Abstract

In the previous paper, the importance of the fluctuation of agglutinin titer was cornfirmed by the author for the serological diagnosis of Salmonella pullorum infection ofadult fowls.
This time, the same fluctuation phenomenon was observed on the hens artificially (intravenously, intraperitoneally or orally) infected with freshly isolated S. pullorum, The results were summarized as follows.
1. The “consistently positive type” and the “undulatory type” of the agglutinin fluctuations were observed regardless of the routes of infection, whether intravenous or intraperitoneal.
2. The sera obtained from three hens, administered orally 1 ml of broth culture of the freshly isolated S. pullorum distinctly developped the fluctuation type of the agglutinin titer and agreed with the “intermittent type” observed in natural cases.
3. Those artificially infected “intermittent type” of S. pullorum carriers showed low agglutinin titers such as 1: 50 or 1: 100, for 15-90 days, and then turned to positive in the consequent tests.