On the Relation between diphtheric Allergy and Antitoxin production

Abstract

1. When persons who were positive in both Shick test and Moloney test, and persons who were positive in Shick test but negative in Moloney test were respectively inoculated with diphtheria toxoid, the former became more readily Shick-negative than the latter.
2. When saline emulsion of boiled and washed cells of diptheria bacilli were inoculated into muscle of rabbits, the skin reaction of the rabbits to Moloney test became positive. This skin reaction was considered as to be allergic, because the specific desensitisation was proved by inoculation of large dosis of saline emulsion of boiled and washed cells of diphtheria bacilli, and the non-spec: flc, by inoculation of chlorpromazine or epinephrine.
3. When the above sensitized rabbits and nonsensitized control rabbits were immunized with diphtheria toxoid, the former became Shick-negative sooner than the latter.
4. The Shick test was proved to remain negative over 159 days after the last inoculation in every cases of the former while it changed to positive at that time in half of the latter.
5. In the neutralization experiment using test by rabbit's skin, it was proved that. the antitoxin titre of sera of the former was higher than the latter, and the rise of the titer of the former was sooner than the latter.
6. When rabbits were immunized with diphtheria toxoid to which a small dosis of L-cystein was added, it was proved that the rise of the antitoxin titre of the rabbits was higher than the titre of control animals.