Studies on Pseudomonas Aeruginosa Infection

I. Biochemical Behaviour of Pseudomonas Aeruginosa

Abstract

The Definition of Pseudomonas aeruginosa studied here is as follows:
Gram-negative motile bacillus, which liquefies gelatin and grows readily at 42°C. on ordinary media. Litmus milk becomes alkaline.
Seventy two strains of Pseudomonas aeruginosa isolated chiefly in our laboratory from various clinical materials were studied in present investigation and it was found that they had the following biochemical behaviour.
1. Activity to carbohydrates in 70 strains, glucose, xylose and arabinose were oxidized, but not fermented.
In 1 strain, glucose, xylose and arabinose were neither oxidized nor fermented In 1 strain, glucose wasre neither oxidized nor fermented, but xylose and arabinose oxidized, not fermented.
Any of rhamnose, lactose, maltose, sucrose, dulcitol and mannitol were not attacked oxidatively or fermentatively.
2. In all Pseudomonas aeruginosa strains, indole was not produced. Methyl red and Voges-Proskauer tests negative. Sodium Citrate utilization positive.
3. Cytochrome oxidase test (Gaby) was positive in all pseudomonas aeruginosa strains.
4. Pyocyanin and fluorescein which were not demonstrable in ordinary agar slant were demonstrated in King's medium A and B, respectively.
Mannitol stimulated the production of pyocyanin.