1964 Volume 38 Issue 3 Pages 57-65
Tho virus isolation and the histo-pathological investigation were carried out by means of Encephalotomy from fatal cases with Japanese B Encephalitis admitted in Kyoto Municipal Hospital since 1960 through 1962.
1) The virus was isolated successfully in suckling mouse brain by intracerebrat inoculation of brain tissue from the fatal patients by means of surgical drill and horseliver punction needle without to open the scalp of the patients.
2) Japanese B Encephalitis virus was detected from the 6 out of the 11 cases. Six cases were positive among 8 cases which died within one week after the onset.
3) The virus isolated from a fatal case was identified by means of HI test against immune guinea pig serum and patient serum.
4) The histo-pathological diagnosis could be made from the brain tissue, in which characteristic findings of encephalitis were noticed, of a patient even who was virus negacive case.
