1975 Volume 49 Issue 1 Pages 12-17
Various samples (human and rat feces, chicken and beef meat) were examined for the presence of Salmonellae employing conventional plating method (BC), ES procedure described in the previous paper and ES cultural method (ESC). The purpose of this paper is to evaluate the ES and ESC procedure for the rapid detection of Salmonellae and to compare the results of these procedures with that of BC procedure.
The results were summarized as follows:
1) Out of 161 samples, 37 strains of Salmonellae from 35 samples were isolated employing 2 cultural methods (BC and ESC). Positive detection rates of Salmonella were 88.6%(31/35), 94.3%(33/35) and 82.9%(29/35), respectively for BC, ESC and ES, However, 2 Salmonella serotypes identified from 2 samples by employing BC and ESC could not be detected by ES, because the antisera used in ES alone could not identify the 2 serotypes.
2) Twenty-six samples were positive, and 123 samples were negative for Salmonella by all of BC, ESC and ES. The results obtained by 3 methods were coincidental by 92.5%.
3) Two Salmonella positive samples were enriched with 24 hours selective enrichment ES pro-cedure, although other 2 positive samples with 5 hours ES procedure turned negative for Salmonella by 24 hours ES procedure. These trends were remarkable in the case of the detection of somatic antigen employing ES procedure. For this reason, 24 hours ES procedure must be performed after the 5 hours ES procedure was finished.
4) ES procedure for the detection of somatic and flagellar antigens appears to be a simpler, specific, rapid and satisfactory method for the detection of Salmonellae.
