1983 Volume 57 Issue 6 Pages 504-511
The superoxide anion (O2-) production by polymorphonuclear leukocytes in 54 diabetics stimulatated by phorbol myristate acetate was determined and compared with those of 25 controls, and the correlation between O2-production and clinical laboratory indices was investigated.
The O2- production in diabetics was 30.04±10.93 (mean±SD, nmol per 4×105 cells) at 10 min., 57.18±16.78 at 30 min., and in controls 38.81 8.11 at 10 min., 69.76 12.60 at 30 min., statistically significant differences were observed between these two groups at both 10 min. (p<0.001) and 30 min. (p<0.01).
The correlation between O2- producton and 8 clinical labolatories indices (age, sex, kinds of therapy, duration of diabetes mellitus, retinopathy, proteinuria, fasting blood glucose value, hemoglobin A1 value) in 54 diabetics was investigated by multivariate analysis. In simple correlation, all clinical laboratory indices were not found to be related to O2- production at either 10 min or 30 min. In partial correlation, however, O production at 10 min possessed a negative correlation with hemoglobin Al value, (p<0.05).
These findings suggest that impaired Oi production might be one of the factors accounting for impaired bactericidal activity of polymorphonuclear leukocytes in diabetics, and that a protracted hyperglycemia might shed some effect on O2- production.
