Studies of the Existence and Distribution of Haemophilus influenzae in the Patients of Chronic Lower Airway Infections by fluorescent Technique

Abstract

H.influenzae is considered to be the most important pathogenic organism in patients with chronic lower airway infections (CLAI).But its existence and distribution of tracheobronchial trees is not clearly recognized.I made antibodies to H.influenzae (non capsulated strain) and examined the existence and distribution of H.influenzae in bronchial tissues of patients with CLAI, by using the fluorescent technique.Subjects were 8 patients with CLAI who were demonstrated H.influenzae in their aspirates obtained by transtracheal aspiration (TTA).As negative control, 2 patients with CLAI demonstrated B. catarrhalis or K. pneumaonia instead of H.influenzae by TTA were examined.Bronchial tissues were obtained from the main bronchus and segmental bronchus (By) of the patients with CLAI by transbronchial bronchial biopsy (TBB).
1.H.influenzae was not detected at main bronchus in all of 8 patient, but was detected at segmental bronchus in eight of all patients.
2.Histologically, H.influenzae was detected in the bronchial secretion and bronchial mucosa in eight of all patients, and in one of them, H.Influenzae was detected in submucosal tissue.
3.H.influenzae was not detected in the case of negative control.
From these results, it is suspected that in the lower airway of patients with CLAI, there is the place where H.influenzae tends to exist.