Sensitivity of Group A Coxsackieviruses and ECHO Viruses, and Isolation form Clinical Specimens by RD-18S Cells Derived by Clorning from RD Cells

Abstract

RD cells can use for Neutralizing antibody test of some Group A Coxsackieviruses (Cox.A). The virus isolation rate from clinical specimens was much lower than from the suckling mice methord.
We carried out the doming of RD cells and had the clorn cell line named RD-18S sensitive to Cox. A viruses.
RD-18S cells were tested for the sensitivity to Enteroviruses and isolation from clinical specimens. The results were as follows.
1) RD-18S cells were sensitive to Cox. A2, 3, 4, 5, 6, 8, 10, 12, 17, 18, 21 and 24 virus and not sensitive to Cox. Al, 19 nor 22 virus.
2) Cytopathic effect (CPE) of RD-18S cells were more clear and sensitive than that of RD cells, especially Cox. A3, 4, 5, 6, 8 and 17 virus.
3) The isolation rate of the Cox. A viruses from clinical specimens was 63.6% in RD-18S and 14.3% in RD cells, compared with that of suckling mice.
4) RD-18S cells were more sensitive than GMK cells to standard ECHO viruses and isolation rate of ECHO viruses from clinical specimens was higher (78.8%) in RD-18S cells than in GMK (40.1%), HEL (70.4%) and HeLa (325.1%) cells.