1988 Volume 62 Issue 11 Pages 1002-1009
A total of 1, 888 patients attending to the obstetrical and gynecological outpatient clinic with various complains were attempted to be diagnosed Chlamydia trachomatis (C. trachomatis) infections by EIA Chlamydiazyme. Furthermore, the serum IgG and IgA titers were measured by ELISA and IFA in 30 Chlamydiazyme and 30 Micro Trak positive cases. 8 cases which were Chlamydiazyme positive and ELISA negative were confirmed by isolation for C. trachomatis using cycloheximide treated HeLa 229 cells.
1) Positive rate in Chlamydiazyme was 9.5% in pregnant women hoping a delivery, 24.2% in prostitutes working in massarge parlor so-called soap-land, 23.1% in students including high school girls.
Positive rate in Chlamydiazyme showed fairly high level than the result in Micro Trak reported by us already.
2) Micro Trak positive cases revealed all positive (16 cases) in ELISA, but Chlamydiazyme was 73.3% in same method.
3) IgA titer over ten times in IFA was 66.7% in Micro Trak positive cases and 53.3% in Chlaamydiazyme positive cases.
4) 8 cases showed positive in Chlamydiazyme and negative in ELISA were completely all negative in isolation. In addition, these cases showed less than 80 times in IFA (IgG) and under 5 times in IFA (IgA).
From these results, we concluded that Chlamydiazyme is a good for the screening of C. trachomatis infection in uterine cervix, but for the final diagnosis should be performed by directimmunofluorescent test as well as Micro Trak. Furthermore, an elevation of sensitivity on the immunofluorescent test is expected, hereafter.
