Two Step Polymerase Chain Reaction法によるLegionella pneumophilaの検出

小出 道夫; 斎藤 厚; 比嘉 太; 山城 祐子; 伊志嶺 朝彦; 普天間 光彦; 稲留 潤; 川上 和義; 草野 展周

doi:10.11150/kansenshogakuzasshi1970.67.1062

Study of Legionella pneumophila Detection by Two Step Polymerase Chain Reaction

Michio KOIDE, Atsushi SAITO, Futoshi HIGA, Yuuko YAMASHIRO, Tomohiko ISHIMINE, Mitsuhiko FUTENMA, Jun INADOME, Kazuyoshi KAWAKAMI, Nobuchika KUSANO

Author information

Michio KOIDE
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus
Atsushi SAITO
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus
Futoshi HIGA
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Yuuko YAMASHIRO
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Tomohiko ISHIMINE
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Mitsuhiko FUTENMA
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Jun INADOME
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Kazuyoshi KAWAKAMI
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus
Nobuchika KUSANO
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus

Keywords: two step PCR, Legionella pneumophila

JOURNAL FREE ACCESS

1993 Volume 67 Issue 11 Pages 1062-1067

DOI https://doi.org/10.11150/kansenshogakuzasshi1970.67.1062

Details

Abstract

We studied theusefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

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