Synergism on the Bactericidal Effect of Gentian Violet (Gv) and Acrinol (Ac) against Pseudomonas aeruginosa

Abstract

The MBCs of Ac against P. aeruginosa (7 strains) isolated from infected skin lesions of patients were more than 6400 μg/ml, and those of Gv were more than 1600 μg/ml. When either Ac or Gv was used independently, these dyes did not have the bactericidal effect of P. aeruginosa. When Gv was used in combination with Ac, predominantly synergism on the bactericidal effect of Ac and Gv against P. aeruginosa was obseved. The MBCs of an Ac-Gv cocktail were between 100 μg/ml and 225 μg/ml. We have previously reported that Gv possessed significantly a bactericidal effect to MRSA isolated from clinical specimens. Therefore, these results suggestedthat a combination treatment by an Ac-Gv cocktail may be one of the useful drugs for the MRSA and P. aeruginosa mixed infection on the skin lesions which is frequently observed clinically.