Isolation and Analysis of Amino Acid Sequence of V3 Region of HIV-1 from Patients with HIV-Infection in Hiroshima

Abstract

Isolation of HIV-1 was tried in PBMCs separated from 27 blood samples which were sent to Hiroshima Prefectural Institute for Health & Environment Science for anti-HIV antibody-test. 18 samples were positive for HIV-isolation (4 samples were Rapid/High type, 12 samples were Slow/Low type and 2 samples were intermediate). Compared with clinical stages of patients, samples from patients in CDC-II were 64%(14/22) positive and those in CDC-IV were 100%(3/3) positive in HIV-isolation. Improvement of the methods is tobe required to isolate HIV from the samples of patient in the latent phase. Removal of CD8 positive cells from PBMCs of patients did not affect the efficiency of HIV-isolation by our methods. Three parts in amino acid sequence of V3 region in proviruses were different between Rapid/High type isolated and Slow/Low type isolates. The difference of amino acid may be related to the different phenotypes of the two viruses.