Molecular Analysis of Streptococcal Pyrogenic Exotoxin Genes among Group A Streptococcus Isolates fram Japanese Patients with Pharyngitis and Skin Infections
2005 Volume 79 Issue 7 Pages 443-450
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2005 Volume 79 Issue 7 Pages 443-450
Group A Streptococci (GAS) from patients with pharyngitis and skin infections were examinedfor Tserotypes, emm types, and streptococcal pyrogenic exotoxin gene types.
The results were summarized as follaws:
1) T and emm types were determined in 130 GAa isolates obtained between 2000 and 2004.Among 85 throat isolates, predominant T/emm types were T12/emm12 (25%), T4/emm4 (19%), andT1/emm1 (14%).Among 45 skin iso1Btes, predominant T/emm types were T28/emm28 (13%), TB3264/emm89 (13%), Tnontypeable/emm58 (13%), T1/emm 1 (11%), and T12/emm (11%).Pre-dominant T/emm types of skin isolates in 2000-2004 slightly differed from those during 1990s in ourprevious report.
2) The presence of streptococcal pyrogenic exotoxin, genes in 292 GAS isolates obtained between1990 and 2004 was examined.Significantly lower proportion of skin isolates, compared with throatisolates, was found to harbar the speA gene (12 versus 26%, respectively;p<0.01), or the speC gene (40 versus 65%, respectively; P< 0.01).All but one of tested isolates carried the speB gene. ThespeB-negative isolate was identified as S. dysgalactiae subsp. equisimilis with the group A antigen.
3) Types of the speA alleles were determined in 59 speA-positive GAS isolates.Among 44 throatisolates, 37 (84%) were speA lineage I (speA1-speA2-speA3-speA6), and 7 (16%) were lineage II (speA4-speA5).Among 15 skin isolates, 11 (73%) were lineage I and 4 (27%) were lineage IL The pair-wise associations were observed between emm type and speA allele: emm1 and speA2, emm3 and speA3, emmd and speA4, emm11 and speA2, emm18 and speA1.
