Abstract
Study was conducted to investigate the synthesis of nascent peptide chains (NPC) on polyribosomes which was reported to be the final destinity of the cell in post-ischemic liver as described by BernelliZazzera, A. (1980).
In D-Galactosamine hepatitis rats, the effects of Glucagon-Insulin (G-I) and prednisolone (PSL) on the synthesis of NPC on free and membrane-bonud polyribosomes were determined, as well as the DNA synthesis and c-AMP contents in liver tissues.
A single dose of G-I elevated maximally the synthesis of NPC on both membrane-bound and free ribosomes 12 and 24 hour after the treatment, respectively. Following the increase in the synthesis of NPC, G-I produced a markedly increase in DNA synthesis in liver tissues 24 hour after the administration.
On the other hand, PSL caused a increase in the synthesis of NPC on polyribosomes only 12 hour after the treatment. However, DNA synthesis was markedly inhibited by PSL.
The administration of G-I produced also a prominent increase in the c-AMP levels of liver tissues as early as 6 hour after the treatment.
These results strongly suggest that synthesis of NPC on polyribosomes plays an important role in repair of cell damage caused by D-Gal and that c-AMP may regulate the synthesis of NPC on polyribosomes and DNA synthesis in liver tissues. Hence, G-I and PSL will prevent the progression of hepatic cell necrosis through increased Synthesis of the NPC on polyribosomes.
