1984 Volume 25 Issue 11 Pages 1433-1443
Isolated hepatocytes were sub-fractionated by low gravity centrifugal technique in a new linear density gradient (1.03-1.09g/ml) of Percoll.
According to their position in the gradient, the cells from fed and fasted rats were divided into four discrete bands, and that from 70% partial hepatectomized rats showed two or three bands.
In order to determine acinar zonal derivation for each fractionated cells, the distribution of fluorescein diacetate in cells fractionated from liver per-perfused was studied, and fractionated cells were examined morphologically by transmission and scanning electron microscopy. It was found that lighter hepatocytes corresponded to the centrilobular cells, whereas heavier hepatocytes represented the periportal cells.
Soluble proteins and activity of glucose-6-phesphatase in fractionated cells were quantitatively measured. The fractionated cells were cultured in medium with serum, and were observed electron microscopically.
