Abstract
We investigated the receptor to Asialoorosomucoid (ASOR) and Agalactorosomucoid (AGOR) on the PLC/PRF/5 cell line. In vivo assay, the athymic mice with tumors which were made by inoculation of the PLC/PRF/5 cells received via the jugular vein 1μg of 125I-ASOR or 125I-AGOR. 10min after injection the accumulation of 1125I-ASOR or 125I-AGOR in the tumor remained only 0.5% of the injected radioactivity. Then, the liver was isolated from the circulation by ligating the portal vein and hepatic artery to increase the ligand concentration in the circulation. And then, the blood concentration of radioactivity was 50 times higher than that in normal athymic mice. There was no enhanced accumulation in the tumor. In monolayer cell culture, the specific uptake of 125I-ASOR and 125I-AGOR on the cells was examined. It was found that the specific uptake of 125I-ASOR was very low (13.7% of total radioactivity bound by the cells) and that of 125I-AGOR was abscent. From these results, it appeared that the receptor to ASOR on PLC/PRF/5 cell line was very low.
