1988 Volume 29 Issue 3 Pages 382-388
Liver sinusoidal large granular lymphocytes (LGL) of Wistar rat were obtained by two step perfusion method via the portal vein. Pre-perfusion was performed with low pressure (below 5 cmH2O) by Ringer's solution, and the second step perfusion was done with high pressure (12-15 cmH2O) by Hanks' balanced.salt solution containing 0.05% collagenase. The perfusate was collected from the hepatic vein. Cellular components of the perfusate were 234.5±95.2×105 mononuclear cells/10g liver (n=4) and the percentage of LGL was 20.9±3.1% (i.e. the yield of LGL was 49.2±21.6×105 cells/10g liver) respectively.
The natural killer activity of liver sinusoidal mononuclear cell, venous blood mononuclear cell and splenic mononuclear cell was studied by 4 hr 51Cr release assay against YAC-1 cell line. % cytotoxicity of liver sinusoidal mononuclear cell was 43.0±5.2% (effector target ratio 20:1) and significantly (p<0.01) higher than those of venous blood and spleen. It was supposed that the liver sinusoidal perfusate contained rich liver-associated LGLs with high natural killer activity.
