Abstract
In order to clarify the cytoprotective role of superoxide dismutase (EC 1.15.1.1 SOD), the localization of Cu, Zn SOD in rat liver tissue following carbon tetrachloride intoxication was investigated, using anti-rat Cu, Zn SOD monoclonal antibody. The antibody was prepared using purified rat Cu, Zn SOD as the antigen. In control liver, Cu, Zn SOD was difusely stained in the lobules. Three different localization patterns of Cu, Zn SOD in hepatocytes could be recognized; cytoplasmic, nuclear, and both cytoplasmic and nuclear localization. Immunoelectron microscopic observation revealed the localization of Cu, Zn SOD in the cytosol matrix, membrane of the rough endoplasmic reticulum and nucleus. Following administration of CCl4, Cu, Zn SOD was strongly localized in ballooning degenerative hepatocytes and present in the cytoplasm of fatty degenerative hepatocytes. Immunoelectron microscopic observation revealed the reaction products localized in the cytosol matrix and on the membrane of rough endoplasmic reticulum around fat droplets. These findings suggest a close relation of superoxide radical to the etiology of liver damage following carbon tetrachloride intoxication.
