Effects of the polysaccharide chain of lipopolysaccharide in an experimental massive hepatic cell necrosis model

Abstract

We have reported that the polysaccharide chain of lipopolysaccharide (LPS) plays an important role in the induction of massive hepatic cell necrosis in an experimental model induced by intravenous injection Propionibacterium acnes (P. acnes) and LPS using mice. In order to elucidate the mechanism by which massive hepatic cell necrosis is induced in our experimental model, we examined whether mouse liver adherent cells produce tumor necrosis factor (TNF), intreleukin 1 (IL1) and hepatocytotoxic factor or not when these cells are treated with various kinds of LPS with different polysaccharide chains. As a result, when LPS from Salmonella minnesota (SM) R345 was administered into 7 days after P. acnes was administered, the production of TNF and hepatocytotoxic factor from liver adherent cells was much compared to when LPS from SM R5 was administered. However, IL1 production was not different between mice administered with LPS from SMR345 and SMR5. These suggested that TNF and hepatocytotoxic factor play a more important role in the induction of massive hepatic cell necrosis in our experimental model compared to IL1.