Detection of serum HCV antibody ELISA assay using to the C-100 synthetic peptide

Abstract

Serum HCV antibody titers in patients with non A non B (NANB) liver diseases were measured by ELISA assay which used chemically-synthesized peptide, 42 amino acid sequence, from the C5-1-1 region of HCV gene. Some of them were compared with those of C-100 antibodies (Chiron Co. Ltd.). Positive rate of serum HCV antibody was 69.3% in NANB chronic hepatitis, 68.5% in chirrosis, 74.4% in hepatic carcinoma, and 20.0% in acute hepatitis. In comparisen with that of C-100 antibodies, the positive rate in this method was low by about 5-10%. It is unknown at percent whether these results are attributable to a pseudpositive response of C-100 antibody or to a low sensitivity of detection in this method. However, this method yielded a high percentage of positive response in NANB liver disease, and seemed useful for the clinical diagnosis of hepatitis C.