Abstract
Burkholderia caryophylli, the pathogen of bacterial wilt of carnation, was detected up to the concentration of 10cfu by PCR and LAMP (Loop-mediated isothermal amplification) using primers based on the domain coding for 16S ribosomal RNA. LAMP was a simple and easy method to detect the bacteria compared with PCR, because LAMP reaction was performed in a shorter time than PCR. The bacteria can be detected by LAMP using materials derived from stuck wooden tooth-pick into the stem of cuttings inoculated with B. caryophilli.
