Abstract
A total of 479 isolates of Botrytis cinerea collected from 2005 to 2009 in Mie Prefecture were tested for their sensitivity to succinate dehydrogenase inhibitor (SDHI) fungicides (Penthiopyrad and Boscalid) by the GLYE-paper disc method or the YBA agar-mycelial disc method. These methods did not match those inoculation tests. However, in the case of the YBA ager-paper disc method, minimum inhibitory concentration (MIC) values of Penthiopyrad and Boscalid fungicides were calculated and 1 ppm than less. The resistant isolate was not detected by the YBA agar-paper disc method (YBA agar medium including SDHI as 1 ppm). Moreover, these results were in accordance with those from the inoculation test on the chemical-sprayed plants. The YBA agar-paper disc method is effective for testing the sensitivity of B. cinerea to Penthiopyrad and Boscalid fungicides.
