Abstract
Ralstonia solanacearum, causing bacterial wilt disease, just after culturing in medium could not be detected in 35 g/L saline solution from 2 days after addition. After keeping R. solanacearum in sterile water for more than 1 month at 25°C, the pathogen survived in 35 g/L saline solution for more than 10 days. For killing the salt-tolerant pathogen, it is necessary to bring it into higher concentrate saline solution. The addition of sugar (glucose or sucrose) into 35 g/L saline solution killed the salt-tolerant pathogen within 4 days. Furthermore, the addition of acids (citric acid or acetic acid), alkali (sodium bicarbonate or sodium carbonate) or surfactant (sodium dodecyl sulfate) into 35 g/L saline solution resulted in killing the salt-tolerant pathogen within 1 day. Especially, addition of 20 mg/L sodium carbonate into saline solution was sufficient to kill the salt-tolerant pathogen within 1 day.
