A simple method to survey a ratio of wheat and barley seeds infested by Pseudomonas syringae pv. syringae using multi-well plate

Abstract

A simple method to survey a ratio of barley or wheat seeds infested by Pseudomonas syringae pv. syringae (Pss). Barley or wheat seeds were put in multi-well plate and immersed in 200 μl sterile water at 8°C for 3 days. A small aliquot of the seed immersed water was transferred on selective medium to detect Pss using 48-pin copy plate, and incubated at 25°C for 7 days. Black colonies of Pss were clearly detected without contamination of filamentous fungi. This is a simple and easy method to examine many seeds at a time, and it is expected to detect infested seeds more precisely.