Detection of Phytophthora nicotianae and Ph. capsici by loop-mediated isothermal amplification assay

Abstract

For the detection of Phytophthora nicotianae and Ph. capsici, a loop-mediated isothermal amplification (LAMP) assay was developed. The LAMP assay using species-specific primers could detect Ph. nicotianae and Ph. capsici within 60 min at 68°C as either extracted DNA, cultured mycelium, or crude sap from inoculated plants. The Multiplex assay using mixed primers and an isothermal DNA amplification and fluorescence detection device could detect Ph. nicotianae and Ph. capsici simultaneously.