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The research on piloti space, which is an important part of building in area with hot and humid summer, has not been studied sufficiently. In this paper, non-isothermal CFD analysis for residential buildings of row layout in Wuhan was carried out using STAR-CD V3.26. Through comparing the results of basic case and four cases with different piloti arrangements, it was clarified that piloti set in the two ends of building is the optimal piloti arrangement. Then the relationship between piloti ratio and wind environment was investigated based on the optimal piloti arrangement. Finally, the wind environments were evaluated using a criteria for assessing wind-induced discomfort considering temperature effect. When piloti ratio is more than 12% in residential area of row layout in Wuhan, the wind-induced comfort can be realized.