KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Lectures
Automated TEM Data Collection for Single Particle Analysis
Natsuko Nakamura
Author information
JOURNALS FREE ACCESS

2010 Volume 45 Issue 1 Pages 42-45

Details
Abstract

Single particle analysis is a TEM application to structural biology. While it has a favorable feature which does not require crystallization of the target particle, it also has an undesirable feature which needs to collect a huge number of data. In the present field, a thin layer of vitreous ice is formed in holes of the supporting film and an appropriate specimen area is selected by DIFF shadow imaging. Their low signal-to-noise ratio and pincushion or barrel distortion prevent automation of searching. An automated data acquisition system JADAS, which can overcome those problems, will be described.

Information related to the author
© 2010 The Japanese Society of Microscopy
Previous article Next article
feedback
Top