KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Lectures
Electron Imaging towards High-Resolution Structure Analysis of Biological Macromolecules
Koji Yonekura
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JOURNALS FREE ACCESS

2010 Volume 45 Issue 4 Pages 243-249

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Abstract

Cryo-electron microscopy (cryo-EM) is now being widely used to analyze biological macromolecular structures because it can be applied to various forms of samples, although attainable resolution is generally not so high.  Helical reconstruction and single particle analysis of highly-symmetrical huge particles have now reached to a resolution better than 4Å, and allowed to visualize densities of amino-acid side chains and build the atomic models directly from the density maps.  In this article, I will introduce examples of high-resolution cryo-EM structures such as the bacterial flagellar filament and a spherical virus, and review imaging conditions towards higher resolutions.  The article also covers new digital imaging devices and energy filtering.

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© 2010 The Japanese Society of Microscopy
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