2014 Volume 49 Issue 3 Pages 176-180
Recently, technologies for three-dimensional reconstruction of biological samples have developed rapidly. Especially, various methods using scanning electron microscopy have emerged. ATUM (Automated Tape-Collecting system Ultra-Microtome) is one of these technologies and ultrathin sections are collected automatically by using ATUM. In this article, I will introduce the detail of ATUM and will describe how to use this equipment, including the application for light microscopy.