Practical Techniques of Cryo-electron Tomography

Abstract

Cryo-electron tomography is a powerful method to visualize the three-dimensional structures of macromolecular complexes and cellular organelles in situ. Compared with the single particle analysis, however, cryo-electron tomography appears to be relatively unpopular probably due to the seemingly complicated methodology. In this review, I will give an overview of the practical aspects of the cryo-electron tomography as well as the recent progress in this field of research.