2023 Volume 58 Issue 2 Pages 71-75
Visualization of the dynamics on the living cell surface is essential for understanding cellular functions such as migration, cancer invasion, and differentiation. In order to evaluate cellular dynamics, it is necessary to develop visualization techniques with nanoscale spatial resolution and non-invasion. Scanning ion conductance microscopy (SICM) is one of the most powerful microscopy techniques for obtaining fragile cell morphology under physiological conditions. In recent years, several research groups have improved the temporal resolution of SICM to visualize rapid cellular reactions. Here, we provide an overview of SICM, including the basic scanning mode and equipment, and recent researches on the development of high-speed SICM.
