2002 Volume 68 Issue 672 Pages 2369-2376
In the present study, effects of iso-osmolar alginate and trehalose solutions on electroporated or unpulsed control murine myeloma cells (Sp2) during the freezing-thawing process are discussed. To investigate the effects of each cryoprotectant, the freezing-thawing process of electroporated or intact cells were observed, which were suspended in alginate-NaCl solution, alginate-trehalose solution or trehalose solution. The microscopic observation took place also at various cooling rates (-1°C/min, -90°C/min, -5°C/min). It was found that extracellular ice grains in the solution containing trehalose did not mechanically suppress cells even at lowest cooling rate. Further, only a seldom intracellular ice nucleation was observed at all cooling rate for the pulsed or unpulsed cells which were suspended in trehalose solution.
Transactions of the Society of Mechanical Engineers
Transactions of the Japan Society of Mechanical Engineers
Transactions of the Japan Society of Mechanical Engineers Series A
TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series C
TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series B
TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series A
Transactions of the Japan Society of Mechanical Engineers Series C
