Differences in Detection of Soybean Dwarf Virus by Enzyme-Linked Immunosorbent Assay (ELISA) in Different Parts of the Ladino Clover

Abstract

Clover in pastures or at the wayside is an influential source of soybean dwarf virus (SDV) infection. Enzyme-linked immunosorbent assay (ELISA) can detect the SDV concentration in plants directly. In this report, differences in SDV concentration in deif f erent parts of the same plant of ladino clover were examined by ELISA. A₄₀₅ values were greatest for the peduncles and fibrous roots. Flower heads, petioles and stolons showed relatively low values. A₄₀₅ values of the leaves were the lowest, presenting the possibility of evaluation as noninfected plants. The most effcient part of ladino clover for SDV detection by ELISA tset, therefore, is the peduncle, because of the high SDV concentration