Abstract
Larvae of Liriomyza huidobrensis (Blanchard) were grown on the kidney bean, Phaseolus vulgaris, under 20°C, 11L-13D. Soon after pupation, which was followed by storage in 10°C for 2 days, the puparia were stored in 0°C for 0, 3, 7, 14, 21, 28, and 35 days. Then they were kept under moderate temperature (25°C) for observation of emergence of the flies. The percentage of emergence decreased after storage in 0°C for more than 7 days and all the puparia died within the 35 days of storage in 0°C. There was no difference in the pre-emergence period under 25°C (ca. 8 days) among the puparia with various duration of the preceding storage in 0°C between 0 and 28 days.
