2024 Volume 74 Issue 1 Pages 43-50
Objectives: MiR-1253, miR-504-5p, miR-26a-5p, miR-21-3p, and miR495-3p have been identified as driver mutation-specific microRNAs (miRNAs) in human non-small-cell lung cancer. We examined the expression of these miRNAs in human lung adenocarcinoma cell lines and investigated whether the miRNAs truly show specific driver mutations.
Material and Methods: We clarified the status of EGFR and KRAS mutations reported in the literature (2 cell lines) and those identified by DNA sequence analysis (7 cell lines) in human lung adenocarcinoma cell lines, and analyzed the expression of the above driver mutation-specific miRNAs in all 9 cell lines.
Results: MiR-504-5p, miR-26a-5p, miR-21-3p, and miR495-3p did not show any expression patterns related to driver mutations in any of the human lung adenocarcinoma cell lines analyzed. On the basis of cytomorphological characteristics, we could not determine the association between driver mutation and morphological changes.
Discussion: The results of this study indicate that the changes in miR-504-5p, miR-26a-5p, miR-21-3p, and miR495-3p were not directly caused by driver mutations that occurred in cancer cells. However, these changes may indicate driver mutation-led transformations in the tumor tissue environment by driver mutation, suggesting that the miRNAs are products of peritumor noncancer cells rather than tumor cells.
