Abstract
Crooke' s chemical method for urinary gonadotropin assay utilizing chromatography was critically examined by reduplication.
1. From non-pregnant women, urinary gonadotropin was extracted by kaolin adsorption method, and change in its value in normal menstrual cycle was determined by an modification of Crooke' s method. The results were compared with those of bioassay, which was performed concurrently, and parallel relation was observed between them. It was consequenently concluded that the Crooke' s method can be applied clinically along with the hitherto used biological method.
2. Urinary gonadotropin from pregnant women were extracted by different methods hitherto used, determined by combination of modified and original Crooke' s method, and examined for change in the course of pregnancy. The results were compared with those by the bioassay, and it was found that GB* obtained by kaolin adsorption method and Crooke's original method was approximately in agreement with change in chorionic gonadotropin in pregnancy and with its biological effects which have been published up to the present.
*GA and GB, designations used by Crooke
