Abstract
The virulent strain of the S. entelitidis was infected intracutaneously to the mice and the course of infection was studied.
1. The least dose, 10-8mg of the microorganisms was enough to kill all the mice infected, and the survival time shortened gradually with the increase of infectious dose.
2. When 10-7mg of the bacteria was infected intracutaneously, they promptly multiplied 100 fold within 24 hours at the infected locus. In the skin, the bacterial growth reached almost the maximum 2 to 3 days after the infection. At the same time the infected bacteria in the local lymph node began to increase and they reached maximum cell density about 5 days after infection. Then the bacteria entered into the blood stream and were taken up by the reticulo-endothellial system. In the systemic infection thus formed, the cell number in the liver increased linearly until they reached about 108/g, when the mice died.
3. The mice died in the intracutaneous infection later than in the intravenous infection. This prolongation may be due to the localization of the infected bacteria within the skin and lymph nodes in the case of the intracutaneous infection.
So in the experimental salmonellosis when infected intracutaneously, it must be considered that the infectious course is separated into the local and the systemic stadium.
