Abstract
1. Venom of Habu snake, Sakishima-habu snake, Hime-habu snake and Mamushi snake were intramuscularly injected into mice, and produced local lesions were compared one another. Further, in vitro test was performed on neutralizing action of α-thiolactoylglycine Naagainst these venoms.
2. All the snake venoms produced acute myodegeneration and necrosis in addition to local hemorrhage, but the venoms of Habu and Sakishima-habu were most virulent, producing, even in a minute dose, lysis and necrosis of muscle fibers. The lesion by the Hime-habu snake venom was mainly hemorrhage, and coagulation necrosis of muscle fibers was observed only in sites of especially severe hemorrhage, which was produced by a dose of above 250γ. Main lesions by the Mamushi snake venom were coagulation and segmentation in all the cases, and myolitic change was relatively slight.
3. Hemorrhagic action of all the snake venom was lost by heating at 100°C for 10 minutes.
4. Hemorrhagic action of all the venoms was inhibited by α-thiolactoylglycine Na, but hemorrhage neutralizing dose of it could not depress edema and myolysis produced by the venoms, of Habu Sakishima-habu, To depress the local lesions produced by heated venoms from these snakes, a sufficiently large dose of this chemical was necessary.
5. From these results of lesions and their neutralization, it was clarified that the above mentioned snake venoms can be divided into two-one causes hemorrhage, accompanied by myodegeration and the other causes myodegeneration, accompanied by interstitial edema. The former was heatlabile, and easily neutralized with SH preparations, while the latter was heatstable, and relatively weakly neutralized with SH preparations.
