IMMUNOCHEMICAL STUDIES ON PHAGE-MEDIATED ANTIGENS IN SALMONELLA

Abstract

1. In Salmonella group A there was no difference in sugar components between S. paratyphi A 1015 [1, 2, 12], which has phage-mediated antigen 1, and S. partyphi A var.durazzo [2, 12], which lacks it. Both strains contained galactose, glucose, mannose, rhamnose, paratose, hexosamine, and N-acetylhexosamine. The antigenic determinant of O-antigen I was Considered to be α-glucosyl- (1→6) -galactose-mannose-. The antigenic determinant of O-antigen 12, whlch is related to the infection of phage iota, was assumed to be α-glucose- (1→4) -galactose-mannose-rhamnose by many workers. However mannose was considered to play an important role as the antigenic determinant of O-antigen 12.
2. In Salmonella group E there was no difference in sugar components between S. anatun [3, 10], S. newington [3, 15], and S. thomasville [(3), (15), 34] which have different phage-mediated antigens. These strains all contained galactose, glucose, mannose, rhamnose, hexosamine, and N-acetyl-hexosamine. The antigenic determinants of O-antigen 10 and 15 were considered to be, respectively, α-galactosyl-mannosyl-rhamnose and β-galactosyl-mannosyl-rhamnose to both of which glucose were linked. In the case of phage conversion from O-antigen 3, 10 to 3, 15, it was considered that the change from α-anomer to β-anomer occurred in the antigenic end-determinant. The antigenic determinant of O-antigen 34 was considered to be α-glucosyl- (1→4) -β-galactosyl-mannosyl-rhamnose. In the case of phage conversion from 3, 15 to (3), (15), 34, it was considered that β-anomer of the galactose as the end determinant in O-antigen 15 was modified by the addition of an α-glucose with 1→4 linkage.
3. In Salmonella group C, galactose, glucose, hexosamine, and N-acetylhexosamine were demonstrated in the polysaccharide of S. kentucky [(8), 20] and galactose, glucose, mannose, rhamnose, abequose, hexosamine, and N-acetylhexosamine were demonstrated in the polysaccharide of S. newport var. puertorico [6, 8]. The antigenic end determinant of phage-mediated antigen 20 was considered to be the sugar like N-acetylglucosamine.