Abstract
1. A Japanese family with Bombay (Oh) phenotype was found. The red cells and saliva of Oh phenotype lacked A, B, H and Leb antigens but had Lea antigen and the serum contained anti-A, B, H and anti-Leb imcomplete antibodies.
2. In the anti-Oh saliva immune chicken serum, the hemagglutinin against Oh and O red cells was found.
The reactivities of Oh saliva and Oh red cells against this hemagglutinin were not destroyed by treatment with α-L-fucosidase from Bac. fulminans, but destroyed with β-galactosidase from Bac. cereus H.
The agglutination of Oh red cells by this antiserum was inhibited strongly by ο-β-D-galactosyl- (1→4)-N-acetyl-D-glucosamine. These results demonstrate that the antigenicity related to the blood group substance of saliva and red cells of Oh phenotype is determined by β-galactosyl residue.
Inhibition capacities of salivas weakened in order of OS, Os, and Oh with quantitative difference though not so much as the difference of H antigen.
