Abstract
Human plasma estrogens were measured in normal females and female patients with mammary cancer before and after menopause.
Plasma were collected at luteal phase. Plasma esteogens (E1 E2 and E3) were extracted by the method of Veldhuis, and purified by alumina column chromatography and thin-layer chromatography. Hitachi 023 gas chromatograph with Lovelock type electron capture was used in present study.
The total estrogens did not varied in both of normal females and patients with mammary cancer.
In both normal females and patients with cancer, the amount of E1 and E2 decreased in tendency before and after menopause, while E3 increased in amount.
Estriol ratio (E3/E1+E2) was slightly higher in the normal females than in the patients with mammary cancer.
