Abstract
The ultrafine cytomorphology of various established cultured cell strains was studied, with special emphasis on the appearance frequency of the nuclear body [NB] in the section plane and its significance. This frequency was higher in strains MKN-45 and GMK-8 than in MKN-28, that is, in poorly differentiated, rather than in well differentiated gastradenocarcinoma-derived strains, the former containing, as well as type 1 NB found in the latter, types 2 and 3 NB's. Human lymphogenic strains showed extremely low frequencies of NB. L-929 cells showed type 1 NB at the rate of 30.3%, type 2 NB at 6.0 % and type 3 NB at 0.8 %, thus making a total as high as 37.1 %. When L cells were morphologically classified into A, B and C types according to the grade of retrograde change, A gave NB 17.7 %, B 45.8 %, and C 25.8 %.The NB of subtype b increased with the advance of retrograde change, the NB frequency being lower in the logarithmic growth phase, increasing in the stationary phase, and again decreasing in the death phase.
