Abstract
From the results of the investigations on the staining features of the eggs laid by female moths of ω1-1 mutant which has been injected with neutral red, nile blue and bismarck brown in several pupal ages, it has been ascertained that the hibernating eggs are stained dark, while the non-hibernating eggs are rather pale. The eggs of the polyvoltine races were also stained rather pale with those dyes, but they were paler than the nonhibernating eggs of the bivoltine. It is seemed that these differences are due to those in the permeability of the dyes into the ovary.
The moths which were injected with the dyes on 5 or 6 days after pupation could lay stained eggs. Those which were injected on 9 or 10 days after pupation laid eggs of two types, stained and unstained. The eggs which were laid early in the oviposition did not be stained with these, while those laid lately were stained. It was clearly observed in the dissected moth that the unstained eggs has already cast off the follicle cells. The experiment shows that the folicle cell of the ova gives the stainability to the colion, which is accelerated by the voltinic hormone secreted from the subesophageal ganglia.
