Abstract
The present experiments were carried out in order to isolate the components of proteins contained in the body fluid of the fifth instar of Bombyx mori L, and to identify the sorts of their proteins, by means of salting out and electrophoresis.
The experimental results may be summarized as follows:
(1) From the fluid above mentioned, seven protein fractions were obtained by salting out with use of (NH4) 2SO4.
(2) Two fractions were successfully obtained in the comparatively pure state, considering from the formation of the single boundary by electrophoresis and from their electrophoretic behaviours and solubilities; the one showed the albumin nature and the other globulin one.
(3) These two proteins were hydrolized respectively, and several amino acids in these hydrolysates were estimated, resulting in a remarkable difference between these two proteins in the content of amino acids.
