Abstract
1. Vitamin B2 concentration in the blood increased gradually during the period of pupation. That of the female pupae reached maximum at the fifth-or sixth-day after pupation at 23°C, and decreased in three or four days after that period. In the male pupae, however, no peak at the fifth-or sixth-day was observable.
2. The VB2concentration in the blood of the female pupae from the second- to the seventh day after pupation was higher than those of males.
3. The VB2concentration in the blood of the female pupae in which both ovaries had been removed out during the fifth larval stage increased grandually, and in the ovariectomized moths they were four times higher than those of the normal female moths.
4. The VB2contents in the eggs laid by the moth, one of whose ovaries had removed out during the fifth larval stage, were higher than those of normal one. The fimilar relations between the normal and the ovariectomized were observable in the measuring of the weight and the number of eggs.
5. The VB2contents in the ovaries increased markedly in the middle pupal stage, and this phenomenon is resemble the mode of the increase of the weight of the ovaries.
6. From these results, it is concluded that considerable amounts of VB2 in the blood are transfered into the ovaries, and this transference is remarkable in the middle pupal stage.
7. There was no marked difference in VB2concentration between the blood of the male pupae which had the implanted ovaries and those of the normal male pupae. However, from the result of measuring the VB2 contents of the ovary implanted into the male body, it was ascertained that VB2was transmitted into the implanted ovary.
