Abstract
Supernatant (3, 500 rpm for 15 min.) of the silkworm blood was fractionated with ammonium sulfate at 1/3, 1/2, 2/3 saturation, or saturation, and each antigenic titer in the precipitation reaction was 102, 400, however, 3, 200 in the original blood. Heating 1/2 saturated or saturated fraction at 56°C for 60 minutes, the antigenic titer did not decrease.
When the electrophoresis employing veronal buffer (pH 8.6, I=0. 1) was performed on filter paper, it was found that migrating (2-3 bands) and not migrating components were contained in the supernatant of the blood. Judging from the patterns of egg albumin, albumin from blood and albumin from wheat, most migrating component seems to be albumin fraction.
Precipitate with ammonium sulfate at 1/3 saturation, considered to be globulin, did not migrate from the starting line and contained small amount of albumin. But in the fraction with ammonium sulfate at 1/2 saturation contained both albumin and globulin, while in the 2/3 saturation or saturation, the amount of albumin increased more than in the fraction at lower saturation.
The ratio of albumin/globulin in the course of development from the larval to pupal stage, there seems to be two fractions in the pupal blood as migrating bands and its color densities were strong. The change was also shown in the migrating bands of the jaundicediseased blood as compared with the normal larval blood. The pattern of the polyhedra (dissolved in 0, 5 per cent Na2CO3 and the resulted supernatant was adjusted to pH 8.6) did not migrate.
