Abstract
An Ina-isolate virus was newly isolated from the flacherie-diseased silkworm larvae which occurred in sericultural farms in Ina City, Nagano Prefecture. The virus has been demonstrated to be quite similar in pathological and chemical natures to the densonucleosis virus of Galleria mellonella and animal parvoviruses. The present study was made to establish the purification method of Ina-isolate virus, and a standard procedure with centrifugation on 50% sucrose and 10-40% sucrose gradient was presented. However, CsCl equilibrium centrifugation to get highly purified virus particles failed to be established. Serological studies with the antiserum of purified Ina-isolate virus revealed that Ina-isolate virus is serologically quite different from an infectious flacherie virus of the silkworm.
