1977 Volume 46 Issue 4 Pages 331-337
The present investigation was undertaken to elucidate the mechanism of sugar transport in pupal ovaries of the silkworm. Developing ovaries were incubated in vitro in modified WYATT's medium containing D-arabinose and other sugars.
The results obtained are as follows:
1. D-arabinose was absorbed into ovaries rapidly during the first 30min and then slowly but significantly by 12hr of incubation.
2. D-arabinose absorption into ovaries exhibited concentration-dependent in 20min incubation and in longer incubation 6hr, the saturation of uptake and the sugar transferred against the concentration gradient.
3. D-arabinose absorption in ovaries was inhibited by glucose but glucose absorption was not inhibited by D-arabinose at all.
4. Some sugars indicated their stereospecificity on the sugar transport in ovaries.
5. D-arabinose absorption was inhibited by NaCN and NaN3.
6. The sugar transport mechanism in pupal ovaries was suggested to be composed as the diffusion process and the active transport system dependent on energy metabolism.